The dgat1 mutant (CS3861) was purchased from TAIR. An abi4‐1 mutant line in a Col‐0 background and an abi5‐1 mutant line in a Ws background were obtained from Dr. Dapeng Zhang. We generated the double mutant (abi4abi5) by crossing the single mutants.

JMJ17-WRKY40 and HY5-ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis TAIR Accession : Locus:2148007: printer-friendly version.

2.2. Transgenic plant generation TheABI4-1345to0andABI5-1097to0promotersequenceswere 2013-01-08 Acts as a negative regulator of abscisic acid (ABA) response during germination through the ubiquitin-mediated proteolysis of ABI5/DPBF1. nucleus Source: TAIR Ref.7 "Characterization of three homologous basic leucine zipper transcription factors (bZIP) of the ABI5 family during Arabidopsis thaliana embryo maturation." Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early BEST Arabidopsis thaliana protein match is: basic leucine-zipper 43 (TAIR:AT5G38800.1); Has 1448 Blast hits to 1448 proteins in 151 species We previously showed that the ahg3-1 mutant has a point mutation in the AtPP2CA gene, which encodes another PP2C that has a major role in the ABA response in seeds (Yoshida et al., 2006b). The levels of AHG1 mRNA were higher in dry seeds and increased during late seed maturation - an expression pattern similar to that of ABI5. Description. IPR043452 is a Plant bZIP transcription factors.

The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA i TAIR Curator Summary Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. TAIR Gene Search Results. ABI5 promotes MFT expression, primarily in the radicle-hypocotyl transition zone and ABI3 suppresses it in the seed. 7 . AT1G55660 : negative regulation of seed germination Source: TAIR "The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity." Piskurewicz U. , Jikumaru Y. , Kinoshita N. , Nambara E. , Kamiya Y. , Lopez-Molina L. Plant Cell 20:2729-2745(2008) [ PubMed ] [ Europe PMC ] [ Abstract ] Arabidopsis, polyclonal, antibody, ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39 Immunogen Recombinant HIS-tagged, full length ABI5 in gel slice, of Arabidopsis thaliana UniProt: Q9SJN0-1, TAIR: At2g36270, overexpressed in E.coli Host Rabbit JMJ17-WRKY40 and HY5-ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis TAIR Accession : Locus:2148007: printer-friendly version. Transgenic lines with similar amounts of ABI5 (En‐2 Super:ABI5‐MYC vs bes1‐D Super:ABI5‐MYC) were selected as a pair, and two independent pairs were selected and used for the following studies (Fig.

ABI5 ABA insensitive 5 RT-PCR Reverse-transcriptase polymerase chain reaction LEA Late embryogenesis abundant genes WT Wild type Introduction Rice that provides staple food for half of the world popula-tion relies heavily on water availability for cultivation.

Abstract ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana). The abscisic acid signal is reported to delay flowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating flowering time is unknown.

These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser42 is critical for ABA regulation of seed germination and gene expression in Arabidopsis. The publisher has kindly granted permission to reproduce this abstract on TAIR. Arabidopsis, polyclonal, antibody, ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39 JMJ17-WRKY40 and HY5-ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis TAIR Accession : Locus:2148007: printer-friendly version. Under these conditions, ABI4 and ABI5 each induced a small set of genes (95 and 59, respectively), with only 16 shared targets.

ABI5 acts as a positive regulator in ABA signaling, regulating both seed germination and post‐germinative seedling development (Finkelstein and Lynch 2000). ABA not only activates ABI5 transcription, but also promotes ABI5 accumulation (Finkelstein and Lynch 2000; Lopez‐Molina et al. 2001).

BioSystems 2013-09-17 2021-03-06 Background ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.

For instance, ABI5 was shown to interact with ABF3 and ABF1 (Kim et al., 2002; Finkelstein et al., 2005).
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TAIR Curator Summary Encodes a nuclear coiled-coil protein related to the carrot peripheral nuclear protein NMCP1 that is involved in the determination of plant nuclear structure. Member of a small gene family in Arabidopsis containing 4 proteins (LNC1-4 or CRWN 1-4) with redundant functions in protection from oxidative damage, control of nuclear morphology and degradation of ABI5. TAIR Curator Summary Encodes a member of the FT and TFL1 family of phosphatidylethanolamine-binding proteins.
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Under these conditions, ABI4 and ABI5 each induced a small set of genes (95 and 59, respectively), with only 16 shared targets. In addition to effector genes involved in seed maturation and storage, several signaling proteins and transcription factors not previously known to be involved in the ABA signaling pathway were identified as targets of ABI4 and/or ABI5.

ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001). Abstract ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana). The abscisic acid signal is reported to delay flowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating flowering time is unknown. The triple mutant had greatly reduced phosphorylation activity in in-gel kinase experiments using basic leucine zipper (bZIP) transcription factors including ABI5.

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Protein Abscisic Acid-insensitive 5. ABA INSENSITIVE 5, F2H17.12, F2H17_12, GIA1, GROWTH-INSENSITIVITY TO ABA 1, AT2G36270

It is expressed in seeds and up-regulated in response to ABA. Loss of function mutants show decreased rate of germination in the presence of ABA. ABA dependent regulation is mediated by both ABI3 and ABI5. 2008-12-16 · Mediates E2-dependent protein ubiquitination. Acts as a negative regulator of abscisic acid signaling. Required for ABI5 degradation, by mediating its ubiquitination. Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses.